9EDB

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State V - dissociated)

Summary for 9EDB

• Entry DOI: 10.2210/pdb9edb/pdb
• Related: 9EAK 9EAL 9ED9 9EDA
• EMDB information: 47943
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, sgRNA, Target Strand, ... (4 entities in total)
• Functional Keywords: crispr-associated endonuclease, dna binding protein, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 4
• Total formula weight: 203540.22

Authors

Kiernan, K.A.,Taylor, D.W. (deposition date: 2024-11-16, release date: 2025-06-18)

• Primary citation: Kiernan, K.A.,Taylor, D.W.; Kinetics-guided cryo-EM enables visualization of Cas9 dissociation post-cleavage; To Be Published,

Experimental method

ELECTRON MICROSCOPY (3.1 Å)