9EBZ
Escherichia coli Carbonic Anhydrase 2 in Space Group C222(1)
Summary for 9EBZ
| Entry DOI | 10.2210/pdb9ebz/pdb |
| Related | 9EAT 9EAW |
| Descriptor | Carbonic anhydrase, ZINC ION (3 entities in total) |
| Functional Keywords | zinc-dependent metalloprotein, lyase |
| Biological source | Escherichia coli BL21(DE3) |
| Total number of polymer chains | 4 |
| Total formula weight | 100784.75 |
| Authors | Rankin, M.R.,Smith, J.L. (deposition date: 2024-11-13, release date: 2024-11-20, Last modification date: 2025-01-29) |
| Primary citation | Rankin, M.R.,Smith, J.L. Serendipitous high-resolution structure of Escherichia coli carbonic anhydrase 2. Acta Crystallogr.,Sect.F, 2025 Cited by PubMed Abstract: X-ray crystallography remains the dominant method of determining the three-dimensional structure of proteins. Nevertheless, this resource-intensive process may be hindered by the unintended crystallization of contaminant proteins from the expression source. Here, the serendipitous discovery of two novel crystal forms and one new, high-resolution structure of carbonic anhydrase 2 (CA2) from Escherichia coli that arose during a crystallization campaign for an unrelated target is reported. By comparing unit-cell parameters with those in the PDB, contaminants such as CA2 can be identified, preventing futile molecular-replacement attempts. Crystallographers can use these new lattice parameters to diagnose CA2 contamination in similar experiments. PubMed: 39812168DOI: 10.1107/S2053230X25000068 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.66 Å) |
Structure validation
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