9EAT

High-Resolution Structure of Escherichia coli Carbonic Anhydrase 2 in Space Group P4(2)2(1)2

9EAT の概要

• エントリーDOI: 10.2210/pdb9eat/pdb
• 分子名称: Carbonic anhydrase 2, ZINC ION (3 entities in total)
• 機能のキーワード: zinc-dependent metalloenzyme, lyase
• 由来する生物種: Escherichia coli BL21(DE3)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25196.19

構造登録者

Rankin, M.R.,Smith, J.L. (登録日: 2024-11-11, 公開日: 2024-11-20, 最終更新日: 2025-02-12)

主引用文献

Rankin, M.R.,Smith, J.L.
Serendipitous high-resolution structure of Escherichia coli carbonic anhydrase 2.
Acta Crystallogr.,Sect.F, 81:47-52, 2025

PubMed Abstract: X-ray crystallography remains the dominant method of determining the three-dimensional structure of proteins. Nevertheless, this resource-intensive process may be hindered by the unintended crystallization of contaminant proteins from the expression source. Here, the serendipitous discovery of two novel crystal forms and one new, high-resolution structure of carbonic anhydrase 2 (CA2) from Escherichia coli that arose during a crystallization campaign for an unrelated target is reported. By comparing unit-cell parameters with those in the PDB, contaminants such as CA2 can be identified, preventing futile molecular-replacement attempts. Crystallographers can use these new lattice parameters to diagnose CA2 contamination in similar experiments.

PubMed: 39812168
DOI: 10.1107/S2053230X25000068

実験手法

X-RAY DIFFRACTION (1.43 Å)