9E2V

Cryo-EM map of homodecameric TraT

これはPDB形式変換不可エントリーです。

9E2V の概要

• エントリーDOI: 10.2210/pdb9e2v/pdb
• EMDBエントリー: 47469
• 分子名称: TraT complement resistance protein (1 entity in total)
• 機能のキーワード: membrane protein involved in surface exclusion, membrane protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 276680.51

構造登録者

Lundgren, C.A.K.,Lea, S.,Deme, J. (登録日: 2024-10-23, 公開日: 2025-04-16, 最終更新日: 2025-12-10)

主引用文献

Chen, N.,Bukys, A.,Lundgren, C.A.K.,Deme, J.C.,El Sayyed, H.,Kapanidis, A.N.,Lea, S.M.,Berks, B.C.
Structure of the conjugation surface exclusion protein TraT.
Commun Biol, 8:1702-1702, 2025

PubMed Abstract: Conjugal transfer of plasmids between bacteria is a major route for the spread of antimicrobial resistance. Many conjugative plasmids encode exclusion systems that inhibit redundant conjugation. In incompatibility group F (IncF) plasmids surface exclusion is mediated by the outer membrane protein TraT. Here we report the cryoEM structure of the TraT exclusion protein complex from the canonical F plasmid of Escherichia coli. TraT is a hollow homodecamer shaped like a chef's hat. In contrast to most outer membrane proteins, TraT spans the outer membrane using transmembrane α-helices. We develop a microscopy-based conjugation assay to probe the effects of directed mutagenesis on TraT. Our analysis provides no support for the idea that TraT has specific interactions with partner proteins. Instead, we infer that TraT is most likely to function by physical interference with conjugation. This work provides structural insight into a natural inhibitor of microbial gene transfer.

PubMed: 41299015
DOI: 10.1038/s42003-025-09102-8

実験手法

ELECTRON MICROSCOPY (2 Å)