9E2J

Variediene synthase with five cyclases

9E2J の概要

• エントリーDOI: 10.2210/pdb9e2j/pdb
• EMDBエントリー: 47454
• 分子名称: Variediene synthase (1 entity in total)
• 機能のキーワード: enzyme, terpene, bifunctional, variediene, transferase
• 由来する生物種: Aspergillus stellatus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 495421.50

構造登録者

Wenger, E.S.,Christianson, D.W. (登録日: 2024-10-22, 公開日: 2025-05-21, 最終更新日: 2025-12-03)

主引用文献

Wenger, E.S.,Christianson, D.W.
Structure of bifunctional variediene synthase yields unique insight on biosynthetic diterpene assembly and cyclization.
Nat Commun, 16:5089-5089, 2025

PubMed Abstract: An unusual family of bifunctional terpene synthases has been identified in which a prenyltransferase assembles 5-carbon precursors to form C geranylgeranyl diphosphate (GGPP), which is then converted into a polycyclic product by a cyclase. Here, we report the cryo-EM structure of a massive, 495-kD bifunctional terpene synthase, variediene synthase from Emericella variecolor (EvVS). The structure reveals a hexameric prenyltransferase core sandwiched between two triads of cyclases. Surprisingly, GGPP is not channeled intramolecularly from the prenyltransferase to the cyclase, but instead is channeled intermolecularly to a non-native cyclase as indicated by substrate competition experiments. These results inform our understanding of carbon management in the greater family of bifunctional terpene synthases, hundreds of which have been identified in fungi. Using sequence similarity networks, we also report the identification of bifunctional terpene synthases in an animal, Adineta steineri, a bdelloid rotifer indigenous to freshwater environments.

PubMed: 40456796
DOI: 10.1038/s41467-025-60537-3

実験手法

ELECTRON MICROSCOPY (3.59 Å)