9DXA

LRRC8A:D Conformation 2

9DXA の概要

• エントリーDOI: 10.2210/pdb9dxa/pdb
• 関連するPDBエントリー: 9DX7
• EMDBエントリー: 47282 47283
• 分子名称: Volume-regulated anion channel subunit LRRC8A,Soluble cytochrome b562, Volume-regulated anion channel subunit LRRC8D, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (3 entities in total)
• 機能のキーワード: ion channel, volume-regulation, membrane protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 634148.75

構造登録者

Lurie, A.,Brohawn, S.G. (登録日: 2024-10-11, 公開日: 2024-11-27, 最終更新日: 2025-12-24)

主引用文献

Lurie, A.,Stephens, C.A.,Kern, D.M.,Henn, K.M.,Latorraca, N.R.,Brohawn, S.G.
Assembly and lipid-gating of LRRC8A:D volume-regulated anion channels.
Nat Commun, 2025

PubMed Abstract: Volume-regulated anion channels (VRACs) are ubiquitously expressed vertebrate ion channels that open in response to hypotonic swelling. VRACs assemble as heteromers of LRRC8A and LRRC8B-E subunits, with different subunit combinations resulting in channels with different properties. Recent studies have described the structures of LRRC8A:C VRACs, but how other VRACs assemble, and which structural features are conserved or variant across channel assemblies remains unknown. Herein, we used cryo-EM to determine structures of a LRRC8A:D VRAC with a 4:2 subunit stoichiometry, which we captured in two conformations. The presence of LRRC8D subunits widens and increases hydrophobicity of the selectivity filter, which may contribute to the unique substrate selectivity of LRRC8D-containing VRACs. The structures reveal lipids bound inside the channel pore, similar to those observed in LRRC8A:C VRACs. We observe that LRRC8D subunit incorporation disrupts packing of the cytoplasmic LRR domains, increasing channel dynamics and opening lateral intersubunit gaps, which we speculate are necessary for pore lipid evacuation and channel activation. Molecular dynamics simulations show that lipids can reside stably within the pore to close the channel. Using electrophysiological experiments, we confirmed that pore lipids block conduction in the closed state, demonstrating that lipid-gating is a general property of VRACs.

PubMed: 41388024
DOI: 10.1038/s41467-025-67052-5

実験手法

ELECTRON MICROSCOPY (3.4 Å)