9DUL

Structure of mutant 30S subunit with extended helix 26, version 4

9DUL の概要

• エントリーDOI: 10.2210/pdb9dul/pdb
• EMDBエントリー: 47169
• 分子名称: 16S rRNA, Small ribosomal subunit protein uS10, Small ribosomal subunit protein uS11, ... (21 entities in total)
• 機能のキーワード: translation, 30s subunit, rrna, ribosomal proteins, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 786067.00

構造登録者

Boyko, K.,Cate, J. (登録日: 2024-10-03, 公開日: 2025-02-05, 最終更新日: 2025-02-19)

主引用文献

Boyko, K.V.,Bernstein, R.A.,Kim, M.,Cate, J.H.D.
Role of Ribosomal Protein bS1 in Orthogonal mRNA Start Codon Selection.
Biochemistry, 64:710-718, 2025

PubMed Abstract: In many bacteria, the location of the mRNA start codon is determined by a short ribosome binding site sequence that base pairs with the 3'-end of 16S rRNA (rRNA) in the 30S subunit. Many groups have changed these short sequences, termed the Shine-Dalgarno (SD) sequence in the mRNA and the anti-Shine-Dalgarno (ASD) sequence in 16S rRNA, to create "orthogonal" ribosomes to enable the synthesis of orthogonal polymers in the presence of the endogenous translation machinery. However, orthogonal ribosomes are prone to SD-independent translation. Ribosomal protein bS1, which binds to the 30S ribosomal subunit, is thought to promote translation initiation by shuttling the mRNA to the ribosome. Thus, a better understanding of how the SD and bS1 contribute to start codon selection could help efforts to improve the orthogonality of ribosomes. Here, we engineered the ribosome to prevent binding of bS1 to the 30S subunit and separate the activity of bS1 binding to the ribosome from the role of the mRNA SD sequence in start codon selection. We find that ribosomes lacking bS1 are slightly less active than wild-type ribosomes in vitro. Furthermore, orthogonal 30S subunits lacking bS1 do not have an improved orthogonality. Our findings suggest that mRNA features outside the SD sequence and independent of binding of bS1 to the ribosome likely contribute to start codon selection and the lack of orthogonality of present orthogonal ribosomes.

PubMed: 39854700
DOI: 10.1021/acs.biochem.4c00688

実験手法

ELECTRON MICROSCOPY (2.56 Å)