9DSE

Cyanide-ligated Bordetella pertussis globin coupled sensor regulatory domain

9DSE の概要

• エントリーDOI: 10.2210/pdb9dse/pdb
• 分子名称: Diguanylate cyclase DosC, PROTOPORPHYRIN IX CONTAINING FE, CYANIDE ION, ... (5 entities in total)
• 機能のキーワード: cooperativity, oxygen-sensing, cyano ligand, heme, signaling protein
• 由来する生物種: Tohama I / ATCC BAA-589 / NCTC 13251
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 59505.41

構造登録者

Hoque, N.J.,Pope, S.R.,Boal, A.K. (登録日: 2024-09-27, 公開日: 2025-12-24, 最終更新日: 2026-01-14)

主引用文献

Hoque, N.J.,Pope, S.R.,Venkatakrishnan, V.,Olori, D.O.,Brady, N.A.,Patterson, D.C.,Anand, G.S.,Liu, Y.,Boal, A.K.,Weinert, E.E.
Cooperative ligand binding in a bacterial heme-based oxygen sensor.
J.Biol.Chem., 302:111025-111025, 2025

PubMed Abstract: Bacteria modulate essential phenotypes in response to external signals such as the availability of molecular oxygen (O). A class of direct O-sensing heme proteins, globin coupled sensors, have been implicated in O-dependent regulation of pathogenic phenotypes including biofilm formation, motility, and virulence. While cooperative O binding is well known in both mammalian and prokaryotic hemoglobins, cooperative ligand binding previously has not been observed in bacterial sensor globins. This study explores the O-dependent allosteric communication between globin domains in the globin-coupled sensor protein from Pectobacterium carotovorum (PccGCS) through equilibrium O binding measurements, X-ray crystallography, resonance Raman spectroscopy, and hydrogen-deuterium exchange mass spectrometry. Based on these experiments, we propose a model of allosteric regulation of O binding that is directed by subtle changes in distal heme pocket protein conformation and transduced through dynamics of helices at the dimer interface of the PccGCS sensor globin. Together this work identifies cooperative ligand binding in a family of bacterial heme proteins, which could allow the bacteria to more robustly respond to small changes in O levels. Furthermore, this work highlights the importance of heme pocket residues in transducing the O binding event within the dimer and suggests a pathway for signal transduction in dimeric myoglobin-like sensor proteins.

PubMed: 41371339
DOI: 10.1016/j.jbc.2025.111025

実験手法

X-RAY DIFFRACTION (2.08 Å)