9DOF

Octahedral small virus-like particles of dengue virus type 2 (local reconstruction)

9DOF の概要

• エントリーDOI: 10.2210/pdb9dof/pdb
• EMDBエントリー: 47082
• 分子名称: glycoprotein E, Protein prM (2 entities in total)
• 機能のキーワード: dengue virus type 2, virus, flavivirus, virus-like particle, prm-e protein, fusion protein, cryoem, virus like particle
• 由来する生物種: dengue virus type 2; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 219646.49

構造登録者

Johnson, A.,Dodes Traian, M.,Walsh, R.M.,Jenni, S.,Harrison, S.C. (登録日: 2024-09-19, 公開日: 2024-12-11, 最終更新日: 2025-03-12)

主引用文献

Johnson, A.,Dodes Traian, M.,Walsh Jr., R.M.,Jenni, S.,Harrison, S.C.
Octahedral small virus-like particles of dengue virus type 2.
J.Virol., 99:e0180924-e0180924, 2025

PubMed Abstract: Flavivirus envelope (E) and precursor M (prM) proteins, when ectopically expressed, assemble into empty, virus-like particles (VLPs). Cleavage of prM to M and loss of the pr fragment converts the VLPs from immature to mature particles, mimicking a similar maturation of authentic virions. Most of the VLPs obtained by prM-E expression are smaller than virions; early, low-resolution cryo-EM studies suggested a simple, 60-subunit, icosahedral organization. We describe here the cryo-EM structure of immature, small VLPs (smVLPs) from dengue virus type 2 and show that they have octahedral rather than icosahedral symmetry. The asymmetric unit of the octahedral particle is an asymmetric trimer of prM-E heterodimers, just as it is on icosahedral immature virions; the full, octahedrally symmetric particle thus has 24 such asymmetric trimers or 72 prM-E heterodimers in all. Cleavage of prM and release of pr generates ovoid, somewhat irregular, mature particles. Previous work has shown that mature smVLPs have fusion properties identical to those of virions, consistent with local, virion-like clustering of 36 E dimers on their surface. The cryo-EM structure and the properties of the smVLPs described here relate directly to ongoing efforts to use them as vaccine immunogens.

PubMed: 39745459
DOI: 10.1128/jvi.01809-24

実験手法

ELECTRON MICROSCOPY (4.24 Å)