9DND

Pseudosymmetric protein nanocage GI4 -F7 (local refinement)

9DND の概要

• エントリーDOI: 10.2210/pdb9dnd/pdb
• EMDBエントリー: 47036
• 分子名称: Pseudosymmetric protein nanocages GI4 A Chain, Pseudosymmetric protein nanocages GI4 C Chain, Pseudosymmetric protein nanocages GI4 B Chain (3 entities in total)
• 機能のキーワード: fusion protein, structural genomics, seattle structural genomics center for infectious disease, ssgcid, pseudosymmetric protein nanocages, viral protein
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94307.78

構造登録者

Park, Y.J.,Dowling, Q.M.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),King, N.P.,Veesler, D. (登録日: 2024-09-17, 公開日: 2024-12-18, 最終更新日: 2025-02-26)

主引用文献

Dowling, Q.M.,Park, Y.J.,Fries, C.N.,Gerstenmaier, N.C.,Ols, S.,Yang, E.C.,Wargacki, A.J.,Dosey, A.,Hsia, Y.,Ravichandran, R.,Walkey, C.D.,Burrell, A.L.,Veesler, D.,Baker, D.,King, N.P.
Hierarchical design of pseudosymmetric protein nanocages.
Nature, 638:553-561, 2025

PubMed Abstract: Discrete protein assemblies ranging from hundreds of kilodaltons to hundreds of megadaltons in size are a ubiquitous feature of biological systems and perform highly specialized functions. Despite remarkable recent progress in accurately designing new self-assembling proteins, the size and complexity of these assemblies has been limited by a reliance on strict symmetry. Here, inspired by the pseudosymmetry observed in bacterial microcompartments and viral capsids, we developed a hierarchical computational method for designing large pseudosymmetric self-assembling protein nanomaterials. We computationally designed pseudosymmetric heterooligomeric components and used them to create discrete, cage-like protein assemblies with icosahedral symmetry containing 240, 540 and 960 subunits. At 49, 71 and 96 nm diameter, these nanocages are the largest bounded computationally designed protein assemblies generated to date. More broadly, by moving beyond strict symmetry, our work substantially broadens the variety of self-assembling protein architectures that are accessible through design.

PubMed: 39695230
DOI: 10.1038/s41586-024-08360-6

実験手法

ELECTRON MICROSCOPY (3.1 Å)