9DNC

Apo crystal structure of RamR variant D2.1

9DNC の概要

• エントリーDOI: 10.2210/pdb9dnc/pdb
• 関連するPDBエントリー: 9DN8 9DN9
• 分子名称: Transcriptional regulator RamR, CHLORIDE ION (3 entities in total)
• 機能のキーワード: directed evolution, transcription factor, stereoselectivity, transcription
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44379.79

構造登録者

Kim, W.,Zhang, Y.J. (登録日: 2024-09-17, 公開日: 2026-04-08, 最終更新日: 2026-08-05)

主引用文献

d'Oelsnitz, S.,Kim, W.,Zhao, N.N.,Hardtke, H.,Ikonomova, S.P.,Alperovich, N.,Vasilyeva, O.,James, M.J.,Zigon, E.S.,Cory, M.B.,Johnson, C.D.,Ellington, A.D.,Justman, Q.A.,Springer, M.,Zhang, Y.J.,Silver, P.A.,Ross, D.
Using enantioselective biosensors to evolve asymmetric biocatalysts.
Nat.Chem.Biol., 2026

PubMed Abstract: Biocatalysts are prized for their enantioselectivity, but slow chromatographic separations required to measure enantiomeric excess bottleneck their development. To overcome this limitation, we evolve enantioselective transcription factors (eTFs) that convert enzyme-catalyzed enantiomer concentrations into programmable gene expression outputs, focusing on imine reductases. Here, using a massively parallel reporter assay, we measure dose-response curves for over 300,000 transcription factor variants in response to an imine precursor and chiral amine products. We quantify the sensitivity, selectivity and dynamic range across variants generated by random, site-saturation and shuffling mutagenesis, isolating variants with exceptional specificity. High-resolution structures of evolved eTFs elucidate how steric effects enforce enantioselectivity, while charge interactions distinguish the imine from the amines. Using two eTFs, we create an ultrahigh-throughput chiral screen to evolve an imine reductase with inverted enantioselectivity. To support generalizability and speed, we design a genetic circuit that enables TF generation within weeks. Our methods enable rapid measurement of asymmetric reactions, supporting innovation in chemical manufacturing.

PubMed: 42493606
DOI: 10.1038/s41589-026-02275-1

実験手法

X-RAY DIFFRACTION (1.9 Å)