9DN0

CryoEM structure of the Strongylocentrotus purpuratus caveolin complex

9DN0 の概要

• エントリーDOI: 10.2210/pdb9dn0/pdb
• EMDBエントリー: 47022
• 分子名称: Caveolin (1 entity in total)
• 機能のキーワード: membrane-shaping protein, monotopic membrane protein, membrane protein
• 由来する生物種: Strongylocentrotus purpuratus (purple sea urchin)
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 197788.95

構造登録者

Connolly, S.M.,Ohi, M.D. (登録日: 2024-09-16, 公開日: 2025-07-09, 最終更新日: 2025-08-20)

主引用文献

Han, B.,Connolly, S.M.,Schultz, D.T.,Wilson, L.F.L.,Gulsevin, A.,Meiler, J.,Karakas, E.,Ohi, M.D.,Kenworthy, A.K.
Evolutionarily diverse caveolins share a common structural framework built around amphipathic disks.
J.Cell Biol., 224:-, 2025

PubMed Abstract: Caveolins are a unique family of membrane remodeling proteins present broadly across animals (Metazoa), and in vertebrates form flask-shaped invaginations known as caveolae. While human caveolin-1 assembles into an amphipathic disk composed of 11 spirally packed protomers, the structural basis underlying caveolin function across animals remains elusive. Here, we predicted structures for 73 caveolins spanning animal diversity, as well as a newly identified choanoflagellate caveolin from Salpingoeca rosetta. This analysis revealed seven conserved structural elements and a propensity to assemble into amphipathic disks. Cryo-EM structures of caveolins from S. rosetta choanoflagellate and the purple sea urchin Strongylocentrotus purpuratus exhibit striking structural similarities to human caveolin-1, validating the structural predictions. Lastly, tracing the chromosomal evolutionary history of caveolins revealed its parahoxozoan ancestral chromosome and evolutionary branches on which caveolins translocated and expanded. These results show that caveolins possess an ancient structural framework predating Metazoa and provide a new structural paradigm to explore the molecular basis of caveolin function across diverse evolutionary lineages.

PubMed: 40772930
DOI: 10.1083/jcb.202411175

実験手法

ELECTRON MICROSCOPY (3.1 Å)