9DJ7

CryoEM structures of yeast cytoplasmic dynein in the presence of ATP and Lis1.

9DJ7 の概要

• エントリーDOI: 10.2210/pdb9dj7/pdb
• 関連するPDBエントリー: 9DI3 9DIU
• EMDBエントリー: 46935
• 分子名称: Dynein heavy chain, cytoplasmic, Nuclear distribution protein PAC1, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: enzyme, aaa protein, motor protein
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 447399.32

構造登録者

Kendrick, A.A.,Leschziner, A.E. (登録日: 2024-09-06, 公開日: 2025-05-28, 最終更新日: 2025-06-04)

主引用文献

Kendrick, A.A.,Nguyen, K.H.V.,Ma, W.,Karasmanis, E.P.,Amaro, R.E.,Reck-Peterson, S.L.,Leschziner, A.E.
Multiple steps of dynein activation by Lis1 visualized by cryo-EM.
Nat.Struct.Mol.Biol., 2025

PubMed Abstract: Cytoplasmic dynein-1 (dynein) is an essential molecular motor controlled in part by autoinhibition. Lis1, a key dynein regulator mutated in the neurodevelopmental disease lissencephaly, plays a role in dynein activation. We recently identified a structure of partially autoinhibited dynein bound to Lis1, which suggests an intermediate state in dynein's activation pathway. However, other structural information is needed to fully understand how Lis1 activates dynein. Here, we used cryo-EM and yeast dynein and Lis1 incubated with ATP at different time points to reveal conformations that we propose represent additional intermediate states in dynein's activation pathway. We solved 16 high-resolution structures, including 7 distinct dynein and dynein-Lis1 structures from the same sample. Our data support a model in which Lis1 relieves dynein autoinhibition by increasing its basal ATP hydrolysis rate and promoting conformations compatible with complex assembly and motility. Together, this analysis advances our understanding of dynein activation and the contribution of Lis1 to this process.

PubMed: 40410592
DOI: 10.1038/s41594-025-01558-w

実験手法

ELECTRON MICROSCOPY (3.8 Å)