9DFY

T-junction triangle 6-6

9DFY の概要

• エントリーDOI: 10.2210/pdb9dfy/pdb
• 分子名称: DNA (5'-D(*CP*AP*GP*CP*AP*GP*CP*CP*TP*GP*AP*AP*TP*A)-3'), DNA (5'-D(P*GP*GP*CP*TP*GP*C)-3'), DNA (50-MER), ... (4 entities in total)
• 機能のキーワード: structure of dna tensegrity triangle with t junctions, design t-6-6, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 23906.41

構造登録者

Koomullam, N.,Mao, C. (登録日: 2024-08-30, 公開日: 2025-11-12, 最終更新日: 2025-12-24)

主引用文献

Li, X.,Siraj, N.,Sha, R.,Mao, C.
Crystallographic Study of DNA T-Junction via Crystal Engineering.
Angew.Chem.Int.Ed.Engl., 64:e18174-e18174, 2025

PubMed Abstract: Engineering DNA crystals is the primary motivation for structural DNA nanotechnology. Among many potential applications, such crystals promise as a platform to precisely (in terms of both location and orientation) organize biomolecules into 3D crystals for X-ray crystallographic studies of the guest biomolecules. The crystal formation depends on rationally designed DNA frameworks instead of unpredictable interactions between the guest molecule themselves; thus, avoiding the crystallization problem of biomolecules. This approach was proposed 40 years ago, however, has not been realized so far. Herein, we report an effort along this direction to study DNA T-junction, a common DNA structure used in DNA nanoconstruction. This study is an initial demonstration of the feasibility of the 40-year-old proposal. We expect that it would be quickly adapted to study many other molecules, particularly nucleic acids (e.g., aptamers, catalytic DNAs/RNAs, ribozymes, and ribonucleoproteins) that are, otherwise, difficult to be studied.

PubMed: 41165523
DOI: 10.1002/anie.202518174

実験手法

X-RAY DIFFRACTION (6.24 Å)