9DDP

E. coli TonB-ExbBD TonB bound to ExbB chain E

9DDP の概要

• エントリーDOI: 10.2210/pdb9ddp/pdb
• EMDBエントリー: 46779
• 分子名称: Biopolymer transport protein ExbB, Protein TonB, Biopolymer transport protein ExbD (3 entities in total)
• 機能のキーワード: ton system, transport protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 196380.67

構造登録者

Celia, H.,Botos, I. (登録日: 2024-08-28, 公開日: 2025-07-09, 最終更新日: 2025-07-16)

主引用文献

Celia, H.,Botos, I.,Ghirlando, R.,Duche, D.,Beach, B.M.,Lloubes, R.,Buchanan, S.K.
Cryo-EM structures of the E. coli Ton and Tol motor complexes.
Nat Commun, 16:5506-5506, 2025

PubMed Abstract: The Ton and Tol motor proteins use the proton gradient at the inner membrane of Gram-negative bacteria as an energy source. The generated force is transmitted through the periplasmic space to protein components associated with the outer membrane, either to maintain the outer membrane integrity for the Tol system, or to allow essential nutrients to enter the cell for Ton. We have solved the high-resolution structures of the E. coli TonB-ExbB-ExbD and TolA-TolQ-TolR complexes, revealing the inner membrane embedded engine parts of the Ton and Tol systems, and showing how TonB and TolA interact with the ExbBD and TolQR subcomplexes. Structural similarities between the two motor complexes suggest a common mechanism for the opening of the proton channel and the propagation of the proton motive force into movement of the TonB and TolA subunits. Because TonB and TolA bind at preferential ExbB or TolQ subunits, we propose a new mechanism of assembly of TonB and TolA with their respective ExbBD and TolQR subcomplexes and discuss its impact on the mechanism of action for the Ton and Tol systems.

PubMed: 40595649
DOI: 10.1038/s41467-025-61286-z

実験手法

ELECTRON MICROSCOPY (3.16 Å)