9DDD

SufS-SufU complex from Mycobacterium Tuberculosis

Summary for 9DDD

• Entry DOI: 10.2210/pdb9ddd/pdb
• Related: 9DCL
• Descriptor: Probable cysteine desulfurase, Possible nitrogen fixation related protein, ALANINE, ... (5 entities in total)
• Functional Keywords: fe-s cluster biogenesis cysteine desulfurase zn binding, biosynthetic protein
• Biological source: Mycobacterium tuberculosis; More
• Total number of polymer chains: 4
• Total formula weight: 125523.67

Authors

Stuteley, S.M.,Bashiri, G. (deposition date: 2024-08-27, release date: 2025-07-30)

Primary citation

Stuteley, S.M.,Chen, J.,Wang, J.,Dawes, S.,Baker, E.N.,Squire, C.J.,Pandelia, M.E.,Bashiri, G.
Feedback regulation of iron-sulfur cluster biogenesis.
Biorxiv, 2025

PubMed Abstract: Iron-sulfur (Fe-S) clusters are ubiquitous cofactors in biological systems. Given their central role in bacterial metabolism and pathogenesis, the biogenesis of Fe-S clusters is tightly controlled. We reveal a feedback regulatory mechanism involving the sulfide producing SufS/SufU complex within the sulfur utilization (SUF) system of , the bacterium that causes tuberculosis. In this mechanism, [2Fe-2S] clusters compete with zinc ions for binding to the sulfide transfer protein SufU. Cluster binding induces SufU tetramerization, which prevents its interaction with the cysteine desulfurase SufS, thereby inhibiting SufS activation and limiting sulfide supply for Fe-S cluster biogenesis. These findings uncover an unrecognized regulatory mechanism in , ensuring strict control of Fe-S cluster production.

PubMed: 40667192
DOI: 10.1101/2025.06.15.659787

Experimental method

X-RAY DIFFRACTION (2 Å)