9DDD

SufS-SufU complex from Mycobacterium Tuberculosis

9DDD の概要

• エントリーDOI: 10.2210/pdb9ddd/pdb
• 関連するPDBエントリー: 9DCL
• 分子名称: Probable cysteine desulfurase, Possible nitrogen fixation related protein, ALANINE, ... (5 entities in total)
• 機能のキーワード: fe-s cluster biogenesis cysteine desulfurase zn binding, biosynthetic protein
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125523.67

構造登録者

Stuteley, S.M.,Bashiri, G. (登録日: 2024-08-27, 公開日: 2025-07-30)

主引用文献

Stuteley, S.M.,Chen, J.,Wang, J.,Dawes, S.,Baker, E.N.,Squire, C.J.,Pandelia, M.E.,Bashiri, G.
Feedback regulation of iron-sulfur cluster biogenesis.
Biorxiv, 2025

PubMed Abstract: Iron-sulfur (Fe-S) clusters are ubiquitous cofactors in biological systems. Given their central role in bacterial metabolism and pathogenesis, the biogenesis of Fe-S clusters is tightly controlled. We reveal a feedback regulatory mechanism involving the sulfide producing SufS/SufU complex within the sulfur utilization (SUF) system of , the bacterium that causes tuberculosis. In this mechanism, [2Fe-2S] clusters compete with zinc ions for binding to the sulfide transfer protein SufU. Cluster binding induces SufU tetramerization, which prevents its interaction with the cysteine desulfurase SufS, thereby inhibiting SufS activation and limiting sulfide supply for Fe-S cluster biogenesis. These findings uncover an unrecognized regulatory mechanism in , ensuring strict control of Fe-S cluster production.

PubMed: 40667192
DOI: 10.1101/2025.06.15.659787

実験手法

X-RAY DIFFRACTION (2 Å)