9DBX

Structure of AG11-2F01 Fab in complex with A/Solomon Islands/3/2006 (H1N1) influenza virus hemagglutinin

9DBX の概要

• エントリーDOI: 10.2210/pdb9dbx/pdb
• EMDBエントリー: 46727
• 分子名称: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, Heavy chain of influenza virus hemagglutinin antibody AG11-2F01, ... (5 entities in total)
• 機能のキーワード: antibody-antigen complex, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 242784.86

構造登録者

Mou, Z.,Lei, R.,Dai, X.,Wu, N. (登録日: 2024-08-23, 公開日: 2025-09-03, 最終更新日: 2025-11-05)

主引用文献

Ouyang, W.O.,Lv, H.,Liu, W.,Lei, R.,Mou, Z.,Pholcharee, T.,Talmage, L.,Tong, M.,Ji, W.,Wang, Y.,Dailey, K.E.,Gopal, A.B.,Choi, D.,Ardagh, M.R.,Rodriguez, L.A.,Guthmiller, J.J.,Dai, X.,Wu, N.C.
High-throughput synthesis and specificity characterization of natively paired influenza hemagglutinin antibodies with oPool + display.
Sci Transl Med, 17:eadt4147-eadt4147, 2025

PubMed Abstract: Antibody discovery is crucial for developing therapeutics and vaccines and for understanding adaptive immunity. However, the lack of approaches to synthesize antibodies with defined sequences in a high-throughput manner represents a major bottleneck in antibody discovery. Here, we present oPool display, a high-throughput cell-free platform that combined oligo pool synthesis and mRNA display to rapidly construct and characterize hundreds to thousands of natively paired antibodies in parallel. As a proof of concept, we applied oPool display to probe the binding specificity of more than 300 uncommon influenza hemagglutinin-specific antibodies against nine hemagglutinin variants through 16 screens. More than 5000 binding tests were performed in 3 to 5 days of hands-on time with further scaling potential. Follow-up structural and functional analysis of two antibodies revealed the versatility of the human immunoglobulin gene segment D3-3 () in recognizing the hemagglutinin stem. Overall, this study established an experimental platform that not only accelerates antibody characterization but also enables unbiased discovery of recurring molecular signatures among antibodies with the same specificity.

PubMed: 40901925
DOI: 10.1126/scitranslmed.adt4147

実験手法

ELECTRON MICROSCOPY (2.9 Å)