9DB0

Molecular basis of pathogenicity of the recently emerged FCoV-23 coronavirus. FCoV-23 S short

9DB0 の概要

• エントリーDOI: 10.2210/pdb9db0/pdb
• 関連するPDBエントリー: 9DAZ
• EMDBエントリー: 46709
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: coronavirus, alphacoronavirus, feline coronavirus, cryo-em, neutralization assays, binding assays, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein
• 由来する生物種: Feline coronavirus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 440980.03

構造登録者

Tortorici, M.A.,Veesler, D.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2024-08-23, 公開日: 2025-07-09, 最終更新日: 2025-09-17)

主引用文献

Tortorici, M.A.,Choi, A.,Gibson, C.A.,Lee, J.,Brown, J.T.,Stewart, C.,Joshi, A.,Harari, S.,Willoughby, I.,Treichel, C.,Leaf, E.M.,Bloom, J.D.,King, N.P.,Tait-Burkard, C.,Whittaker, G.R.,Veesler, D.
Loss of FCoV-23 spike domain 0 enhances fusogenicity and entry kinetics.
Nature, 645:235-243, 2025

PubMed Abstract: The ability of coronaviruses to recombine and cross species barriers affects human and animal health globally and is a pandemic threat. FCoV-23 is a recently emerged, highly pathogenic recombinant coronavirus responsible for a widespread outbreak of feline infectious peritonitis. Here we report cryogenic electron microscopy structures of two FCoV-23 spike isoforms that correspond to the in-host loss of domain 0 observed in clinical samples. The loss of domain 0 markedly enhances the fusogenicity and kinetics of entry into cells and possibly enables biotype switching and lethality. We show that FCoV-23 can use several aminopeptidase N orthologues as receptors and reveal the molecular determinants of receptor species tropism, including a glycan that modulates human receptor engagement. We define antigenic relationships among alphacoronaviruses that infect humans and other mammalian species and identify a cross-reactive alphacoronavirus monoclonal antibody that inhibits FCoV-23 entry. Our results pave the way for the development of vaccines and therapeutics that target this highly pathogenic virus.

PubMed: 40634609
DOI: 10.1038/s41586-025-09155-z

実験手法

ELECTRON MICROSCOPY (2.5 Å)