9D80

Shigella flexneri bacteriophage Moo19 Tail

9D80 の概要

• エントリーDOI: 10.2210/pdb9d80/pdb
• EMDBエントリー: 46623
• 分子名称: Portal protein, Gp83, Tail protein, ... (4 entities in total)
• 機能のキーワード: moo19, virus
• 由来する生物種: Shigella virus Moo19; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 496641.56

構造登録者

Subramanian, S.,Bergland Drarvik, S.M.,Parent, K.N. (登録日: 2024-08-18, 公開日: 2025-01-15)

主引用文献

Subramanian, S.,Bergland Drarvik, S.M.,Tinney, K.R.,Doore, S.M.,Parent, K.N.
Moo19 and B2: Structures of Schitoviridae podophages with T = 9 geometry and tailspikes with esterase activity.
Sci Adv, 10:eadt0022-eadt0022, 2024

PubMed Abstract: Podophages are, by far, the least well studied of all the bacteriophages. Despite being classified together due to their short, noncontractile tails, there is a huge amount of diversity among members of this group. Of the podophages, the N4-like family is the least well studied structurally and is quite divergent from well-characterized podophages such as T7 and P22. In this work, we isolate and fully characterize two members of the family by cryo-electron microscopy, genetics, and biochemistry. We describe the capsid features of Moo19 and B2, including a decoration protein. In addition, we have fully modeled the tail machinery for both phages and identify proteins with esterase activity. Genetic knockouts of the host reveal factors specific for host attachment including key modifications to the O-antigen on the lipopolysaccharide. Moo19 and B2 are both members, yet some distinct differences in the genome and structure place them into distinct clades.

PubMed: 39693418
DOI: 10.1126/sciadv.adt0022

実験手法

ELECTRON MICROSCOPY (3.7 Å)