9D2Y

Cryo-EM structure of mycocerosic acid synthase with double KS-ACP crosslinking using C16 alpha-bromoamide. Complex A

これはPDB形式変換不可エントリーです。

9D2Y の概要

• エントリーDOI: 10.2210/pdb9d2y/pdb
• EMDBエントリー: 46504
• 分子名称: Multifunctional mycocerosic acid synthase membrane-associated MAS, C16 alpha-bromoamide (2 entities in total)
• 機能のキーワード: fas, polyketide, crosslinked, ketosynthase, biosynthetic protein
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 453580.55

構造登録者

Heberlig, G.W.,Jiang, Z.,Burkart, M.D. (登録日: 2024-08-09, 公開日: 2025-08-13, 最終更新日: 2025-09-03)

主引用文献

Jiang, Z.,Heberlig, G.W.,Chen, J.A.,Huynh, J.,La Clair, J.J.,Burkart, M.D.
Visualizing acyl carrier protein interactions within a crosslinked type I polyketide synthase.
Nat Commun, 16:7798-7798, 2025

PubMed Abstract: Using a combination of dual covalent crosslinking and cryo-EM analyses, we elucidate the structure of mycocerosic acid synthase from Mycobacterium tuberculosis trapped in two distinct catalytic states during its iterative cycle. These structures reveal domain architecture of the acyl carrier protein mediating condensation and dehydration through dual site-selective crosslinking of the acyl carrier protein with the ketosynthase and dehydratase domains. Map density was sufficient to visualize full domain architecture with active site-bound probes and elucidate key interactions of four distinct crosslinked species. Here, iterative vectorial polyketide biosynthesis arises through an overall twisting and tilting architecture, enabling positioning and entry of the cognate substrate at each enzymatic domain. These structures present valuable details for future therapeutic design against mycocerosic acid biosynthesis in M. tuberculosis.

PubMed: 40841798
DOI: 10.1038/s41467-025-63024-x

実験手法

ELECTRON MICROSCOPY (3.87 Å)