9D2F

3D structure and atomic model of RS3 of mouse respiratory cilia

これはPDB形式変換不可エントリーです。

9D2F の概要

• エントリーDOI: 10.2210/pdb9d2f/pdb
• EMDBエントリー: 46494
• 分子名称: Adenylate kinase 9, Cilia- and flagella-associated protein 43, Cilia- and flagella-associated protein 44, ... (25 entities in total)
• 機能のキーワード: axoneme, radial spoke, structural protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 29
• 化学式量合計: 3090152.63

構造登録者

Yanhe, Z.,Xuewu, Z.,Daniela, N. (登録日: 2024-08-08, 公開日: 2025-08-13, 最終更新日: 2026-02-25)

主引用文献

Zhao, Y.,Song, K.,Tavakoli, A.,Gui, L.,Fernandez-Gonzalez, A.,Zhang, S.,Dzeja, P.P.,Mitsialis, S.A.,Zhang, X.,Nicastro, D.
Mouse radial spoke 3 is a metabolic and regulatory hub in cilia.
Nat.Struct.Mol.Biol., 32:1542-1554, 2025

PubMed Abstract: Cilia are microtubule-based organelles that have important roles in cell sensing, signaling and motility. Recent studies have revealed the atomic structures of many multicomponent ciliary complexes, elucidating their mechanisms of action. However, little is known about the structure, proteome and function of full-length radial spoke 3 (RS3), a conserved complex that transmits mechanochemical signals to coordinate ciliary motility. Here, we combined single-particle cryo-electron microscopy, cryo-electron tomography, proteomic analysis and computational modeling to determine the three-dimensional structure and atomic model of RS3 from mouse respiratory cilia. We reveal all RS3 components, including regulatory and metabolic enzymes such as a protein kinase A subunit, adenylate kinases (AKs) and malate dehydrogenases. Furthermore, we confirm RS3 loss in AK7-deficient mice, which exhibit motility defects. Our findings identify RS3 as an important regulatory and metabolic hub that maintains sufficient adenosine triphosphate for sustained ciliary beating, providing insights into the etiology of ciliopathies.

PubMed: 40579595
DOI: 10.1038/s41594-025-01594-6

実験手法

ELECTRON MICROSCOPY (4.7 Å)