9D10

Photoactive Yellow Protein, crystals from PEG, room temperature

Summary for 9D10

• Entry DOI: 10.2210/pdb9d10/pdb
• Descriptor: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (3 entities in total)
• Functional Keywords: photoactive yellow protein, phototaxis, signaling protein
• Biological source: Halorhodospira halophila
• Total number of polymer chains: 1
• Total formula weight: 14052.73

Authors

Malla, T.N.,Schmidt, M. (deposition date: 2024-08-07, release date: 2025-01-15, Last modification date: 2025-01-22)

Primary citation

Malla, T.N.,Muniyappan, S.,Menendez, D.,Ogukwe, F.,Dale, A.N.,Clayton, J.D.,Weatherall, D.D.,Karki, P.,Dangi, S.,Mandella, V.,Pacheco, A.A.,Stojkovic, E.A.,Rose, S.L.,Orlans, J.,Basu, S.,de Sanctis, D.,Schmidt, M.
Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.
Iucrj, 12:36-48, 2025

PubMed Abstract: The upgrade of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France to an Extremely Brilliant Source (EBS) is expected to enable time-resolved synchrotron serial crystallography (SSX) experiments with sub-millisecond time resolution. ID29 is a new beamline dedicated to SSX experiments at ESRF-EBS. Here, we report experiments emerging from the initial phase of user operation at ID29. We first used microcrystals of photoactive yellow protein as a model system to exploit the potential of microsecond pulses for SSX. Subsequently, we investigated microcrystals of cytochrome c nitrite reductase (ccNiR) with microsecond X-ray pulses. CcNiR is a decaheme protein that is ideal for the investigation of radiation damage at the various heme-iron sites. Finally, we performed a proof-of-concept subsecond time-resolved SSX experiment by photoactivating microcrystals of a myxobacterial phytochrome.

PubMed: 39575537
DOI: 10.1107/S2052252524010868

Experimental method

X-RAY DIFFRACTION (1.8 Å)