9CXT

Hemagglutinin A/Hong Kong/1/68 produced in GnTI- cells

9CXT の概要

• エントリーDOI: 10.2210/pdb9cxt/pdb
• EMDBエントリー: 45997
• 分子名称: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain,Green fluorescent protein fusion, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: hemagglutinin, glycoprotein, viral protein
• 由来する生物種: Influenza A virus (strain A/Hong Kong/1/1968 H3N2); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 287773.45

構造登録者

Torrents de la Pena, A.,de Paiva Froes Rocha, R.,Ward, A.B. (登録日: 2024-07-31, 公開日: 2025-08-06, 最終更新日: 2026-01-07)

主引用文献

de Paiva Froes Rocha, R.,Tomris, I.,Bowman, C.A.,Stevens, E.,Kantorow, J.,Plitt, C.M.,Peng, W.,Oeverdieck, S.,Galdino Andrade, T.,Ferguson, J.A.,Jung, D.D.,Marques, R.E.,Herfst, S.,Snijder, J.,Chakraborty, S.,Torrents de la Pena, A.,Berndsen, Z.T.,de Vries, R.P.,Ward, A.B.
Structural and immunological characterization of the H3 influenza hemagglutinin during antigenic drift.
Nat Commun, 16:11452-11452, 2025

PubMed Abstract: The quest for a universal influenza vaccine holds great promise for mitigating the global burden of influenza-related morbidity and mortality. However, challenges persist in identifying conserved epitopes capable of eliciting robust and durable immune responses. In this study, we explore the influence of glycan evolution on H3 hemagglutinin from 1968 to present day and its impacts on protein structure, antigenicity and immunogenicity by using computational, biochemical and biophysical techniques. Structural characterization of HK/68 and Sing/16 by cryo-electron microscopy shows that while HK/68 is resistant to enzymatic deglycosylation, removal of glycans destabilizes the hyperglycosylated head and membrane-proximal region in Sing/16. Furthermore, the appearance of glycans in Sing/16 hemagglutinin head domain shifts the polyclonal immune response upon vaccination to target the esterase and stem. These insights expand our understanding of glycans beyond their role in protein folding and highlight the interplay among glycan integration and immune recognition to design a universal influenza vaccine.

PubMed: 41381528
DOI: 10.1038/s41467-025-66375-7

実験手法

ELECTRON MICROSCOPY (3.4 Å)