9CS7

Structure of Azospirillum bacterial CARD crystal form 1

9CS7 の概要

• エントリーDOI: 10.2210/pdb9cs7/pdb
• 分子名称: Serine protease (2 entities in total)
• 機能のキーワード: anti-phage defense, caspase activation and recruitment domain, immune system
• 由来する生物種: Azospirillum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23304.38

構造登録者

Wein, T.,Millman, A.,Lange, K.,Yirmiya, E.,Hadary, R.,Garb, J.,Melamed, S.,Steinruecke, F.,HIll, A.B.,Kranzusch, P.J.,Sorek, R. (登録日: 2024-07-23, 公開日: 2025-01-29, 最終更新日: 2025-03-26)

主引用文献

Wein, T.,Millman, A.,Lange, K.,Yirmiya, E.,Hadary, R.,Garb, J.,Melamed, S.,Amitai, G.,Dym, O.,Steinruecke, F.,Hill, A.B.,Kranzusch, P.J.,Sorek, R.
CARD domains mediate anti-phage defence in bacterial gasdermin systems.
Nature, 639:727-734, 2025

PubMed Abstract: Caspase recruitment domains (CARDs) and pyrin domains are important facilitators of inflammasome activity and pyroptosis. Following pathogen recognition by nucleotide binding-domain, leucine-rich, repeat-containing (NLR) proteins, CARDs recruit and activate caspases, which, in turn, activate gasdermin pore-forming proteins to induce pyroptotic cell death. Here we show that CARD domains are present in defence systems that protect bacteria against phage. The bacterial CARD domain is essential for protease-mediated activation of certain bacterial gasdermins, which promote cell death once phage infection is recognized. We further show that multiple anti-phage defence systems use CARD domains to activate a variety of cell death effectors, and that CARD domains mediate protein-protein interactions in these systems. We find that these systems are triggered by a conserved immune-evasion protein used by phages to overcome the bacterial defence system RexAB, demonstrating that phage proteins inhibiting one defence system can activate another. Our results suggest that CARD domains represent an ancient component of innate immune systems conserved from bacteria to humans, and that CARD-dependent activation of gasdermins is shared in organisms across the tree of life.

PubMed: 39880956
DOI: 10.1038/s41586-024-08498-3

実験手法

X-RAY DIFFRACTION (1.88 Å)