9CJZ

CryoEM structure of NC99 hemagglutinin trimer in complex with Fab T009 3-E04

9CJZ の概要

• エントリーDOI: 10.2210/pdb9cjz/pdb
• EMDBエントリー: 45637
• 分子名称: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, 3-E04 Heavy chain, ... (4 entities in total)
• 機能のキーワード: immune system, hemagglutinin, fab, macaque, viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 259048.15

構造登録者

Li, N.,Tsybovsky, Y.,Sangesland, M.,Kanekiyo, M. (登録日: 2024-07-08, 公開日: 2025-07-09)

主引用文献

Sangesland, M.,Li, N.,Tsybovsky, Y.,Rodgers, M.D.,Han, J.,Rodriguez, A.J.,Ferguson, J.A.,Henry, A.R.,Smith, S.C.,Roberts-Torres, J.,Gillespie, R.A.,Liu, C.,Merriam, J.S.,Stephens, T.,Williams, C.,Maestle, E.,Corcoran, M.,Ravichandran, M.,Creanga, A.,Andrews, S.F.,Pierson, T.C.,Karlsson Hedestam, G.B.,Schramm, C.A.,Reed, D.S.,Douek, D.C.,Zhou, T.,Ward, A.B.,Kanekiyo, M.
Functional, Immunogenetic, and Structural Convergence in Influenza Immunity between Humans and Macaques.
Biorxiv, 2025

PubMed Abstract: Human B cell immunity to the influenza hemagglutinin (HA) stem region, a universal influenza vaccine target, is often stereotyped and immunogenetically restricted, posing challenges for study outside humans. Here, we show that macaques vaccinated with a HA stem immunogen elicit human-like public B cell lineages targeting two major conserved sites of vulnerability, the central stem and anchor epitopes. Central stem antibodies were predominantly derived from V1-138, the macaque homolog of human V1-69, a V-gene preferentially used in human central stem broadly neutralizing antibodies (bnAbs). Similarly, macaques produced anchor bnAbs with the human-like NWP motif. Both bnAb lineages were functionally and structurally analogous to their human counterparts, with recognition mediated largely by germline-encoded motifs. Thus the macaque immunoglobulin repertoire supports human-like public bnAb responses to influenza HA. Moreover, this underscores the utility of homologous germline-encoded immunity, suggesting that immune repertoires of macaques and humans may have been similarly shaped during evolution.

PubMed: 40568173
DOI: 10.1101/2025.02.21.639368

実験手法

ELECTRON MICROSCOPY (3.8 Å)