9CJY
CryoEM structure of NC99 hemagglutinin trimer in complex with Fab BB798E 3-C07
Summary for 9CJY
| Entry DOI | 10.2210/pdb9cjy/pdb |
| EMDB information | 45636 |
| Descriptor | Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, 3-C07 Heavy chain, ... (4 entities in total) |
| Functional Keywords | immune system, hemagglutinin, fab, macaque, viral protein |
| Biological source | Influenza A virus More |
| Total number of polymer chains | 12 |
| Total formula weight | 259084.59 |
| Authors | Li, N.,Tsybovsky, Y.,Sangesland, M.,Kanekiyo, M. (deposition date: 2024-07-08, release date: 2025-07-09) |
| Primary citation | Sangesland, M.,Li, N.,Tsybovsky, Y.,Rodgers, M.D.,Han, J.,Rodriguez, A.J.,Ferguson, J.A.,Henry, A.R.,Smith, S.C.,Roberts-Torres, J.,Gillespie, R.A.,Liu, C.,Merriam, J.S.,Stephens, T.,Williams, C.,Maestle, E.,Corcoran, M.,Ravichandran, M.,Creanga, A.,Andrews, S.F.,Pierson, T.C.,Karlsson Hedestam, G.B.,Schramm, C.A.,Reed, D.S.,Douek, D.C.,Zhou, T.,Ward, A.B.,Kanekiyo, M. Functional, Immunogenetic, and Structural Convergence in Influenza Immunity between Humans and Macaques. Biorxiv, 2025 Cited by PubMed Abstract: Human B cell immunity to the influenza hemagglutinin (HA) stem region, a universal influenza vaccine target, is often stereotyped and immunogenetically restricted, posing challenges for study outside humans. Here, we show that macaques vaccinated with a HA stem immunogen elicit human-like public B cell lineages targeting two major conserved sites of vulnerability, the central stem and anchor epitopes. Central stem antibodies were predominantly derived from V 1-138, the macaque homolog of human V 1-69, a V -gene preferentially used in human central stem broadly neutralizing antibodies (bnAbs). Similarly, macaques produced anchor bnAbs with the human-like NWP motif. Both bnAb lineages were functionally and structurally analogous to their human counterparts, with recognition mediated largely by germline-encoded motifs. Thus the macaque immunoglobulin repertoire supports human-like public bnAb responses to influenza HA. Moreover, this underscores the utility of homologous germline-encoded immunity, suggesting that immune repertoires of macaques and humans may have been similarly shaped during evolution. PubMed: 40568173DOI: 10.1101/2025.02.21.639368 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
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