9CE9
Solution NMR structure of the fungal loosenin PcaLOOL12 from Phanerochaete carnosa
Summary for 9CE9
| Entry DOI | 10.2210/pdb9ce9/pdb |
| NMR Information | BMRB: 31180 |
| Descriptor | RlpA-like protein double-psi beta-barrel domain-containing protein (1 entity in total) |
| Functional Keywords | expansin, non-lytic, cellulose, carbohydrate-active enzymes, defibrillation, sugar binding protein |
| Biological source | Phanerochaete carnosa (strain HHB-10118-sp) (Phanerochaete carnosa (strain HHB-10118-sp)) |
| Total number of polymer chains | 1 |
| Total formula weight | 10779.80 |
| Authors | Buchko, G.W.,Master, E.R. (deposition date: 2024-06-26, release date: 2024-07-10, Last modification date: 2025-03-19) |
| Primary citation | Dahiya, D.,Peter-Szabo, Z.,Senanayake, M.,Pingali, S.V.,Leite, W.C.,Byrnes, J.,Buchko, G.W.,Sivan, P.,Vilaplana, F.,Master, E.R.,O'Neill, H. SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates. Biotechnol Biofuels Bioprod, 18:27-27, 2025 Cited by PubMed Abstract: Microbial expansin-related proteins include fungal loosenins, which have been previously shown to disrupt cellulose networks and enhance the enzymatic conversion of cellulosic substrates. Despite showing beneficial impacts to cellulose processing, detailed characterization of cellulosic materials after loosenin treatment is lacking. In this study, small-angle neutron scattering (SANS) was used to investigate the effects of three recombinantly produced loosenins that originate from Phanerochaete carnosa, PcaLOOL7, PcaLOOL9, and PcaLOOL12, on the organization of holocellulose preparations from Eucalyptus and Spruce wood samples. PubMed: 40022179DOI: 10.1186/s13068-025-02618-5 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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