9CD4

Cryo-EM structure of Candidatus Saccharibacterium phosphoketolase complexed with 2-acetyl-thiamine diphosphate

9CD4 の概要

• エントリーDOI: 10.2210/pdb9cd4/pdb
• 関連するPDBエントリー: 9CD3
• EMDBエントリー: 45461 45462
• 分子名称: Phosphoketolase family protein, 2-ACETYL-THIAMINE DIPHOSPHATE (2 entities in total)
• 機能のキーワード: acetyl-phosphate synthase, glycolaldehyde dehydration, carbohydrate metabolic process, aldehyde-lyase activity, lyase
• 由来する生物種: Candidatus Saccharibacteria bacterium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 181549.03

構造登録者

Singal, B.,Landwehr, G.,Jewett, M.C. (登録日: 2024-06-24, 公開日: 2025-08-06, 最終更新日: 2025-11-26)

主引用文献

Landwehr, G.M.,Vogeli, B.,Tian, C.,Singal, B.,Gupta, A.,Lion, R.,Sargent, E.H.,Karim, A.S.,Jewett, M.C.
A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates.
Biorxiv, 2024

PubMed Abstract: Biotechnological processes hold tremendous potential for the efficient and sustainable conversion of one-carbon (C1) substrates into complex multi-carbon products. However, the development of robust and versatile biocatalytic systems for this purpose remains a significant challenge. In this study, we report a hybrid electrochemical-biochemical cell-free system for the conversion of C1 substrates into the universal biological building block acetyl-CoA. The synthetic reductive formate pathway (ReForm) consists of five core enzymes catalyzing non-natural reactions that were established through a cell-free enzyme engineering platform. We demonstrate that ReForm works in a plug-and-play manner to accept diverse C1 substrates including CO equivalents. We anticipate that ReForm will facilitate efforts to build and improve synthetic C1 utilization pathways for a formate-based bioeconomy.

PubMed: 39149402
DOI: 10.1101/2024.08.08.607227

実験手法

ELECTRON MICROSCOPY (2.3 Å)