9CCS

CryoEM Structure of Escherichia coli FimCH in complex with 2H04 Fab

9CCS の概要

• エントリーDOI: 10.2210/pdb9ccs/pdb
• EMDBエントリー: 45456
• 分子名称: Type 1 fimbiral adhesin FimH, 2H04 Fab light chain, 2H04 Fab heavy chain (3 entities in total)
• 機能のキーワード: adhesin, inhibitor, complex, fab, chaperone, usher, pili, cell adhesion
• 由来する生物種: Escherichia coli UTI89; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 79324.18

構造登録者

Lopatto, E.D.B.,Hultgren, S.J. (登録日: 2024-06-23, 公開日: 2025-07-02)

主引用文献

Lopatto, E.D.B.,Santiago-Borges, J.M.,Sanick, D.A.,Malladi, S.K.,Azimzadeh, P.N.,Timm, M.W.,Fox, I.F.,Schmitz, A.J.,Turner, J.S.,Sayed Ahmed, S.M.,Ortinau, L.,Gualberto, N.C.,Pinkner, J.S.,Dodson, K.W.,Ellebedy, A.H.,Kau, A.L.,Hultgren, S.J.
Monoclonal antibodies targeting the FimH adhesin protect against uropathogenic E. coli UTI.
Sci Adv, 11:eadw0698-eadw0698, 2025

PubMed Abstract: As antimicrobial resistance increases, urinary tract infections (UTIs) are expected to pose an increased burden in morbidity and expense on the health care system, increasing the need for alternative antibiotic-sparing treatments. Most UTIs are caused by uropathogenic (UPEC), whereas causes a large portion of non-UPEC UTIs. Both bacteria express type 1 pili tipped with the mannose-binding FimH adhesin critical for UTI pathogenesis. We generated and biochemically characterized 33 murine monoclonal antibodies (mAbs) to FimH. Three mAbs protected mice from UTI. Mechanistically, we show that this protection is Fc independent and mediated by the ability of these mAbs to sterically block FimH function by recognizing a high-affinity FimH conformation. Our data reveal that FimH mAbs hold promise as an antibiotic-sparing treatment strategy.

PubMed: 40540557
DOI: 10.1126/sciadv.adw0698

実験手法

ELECTRON MICROSCOPY (3.17 Å)