9CAU

DeltaArp8 INO80 bound to S.c 0/40 nucleosome, Nucleosome

9CAU の概要

• エントリーDOI: 10.2210/pdb9cau/pdb
• EMDBエントリー: 45404
• 分子名称: Histone H3, Histone H4, Histone H2A.1, ... (6 entities in total)
• 機能のキーワード: chromatin remodeler, nucleosome, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 250346.09

構造登録者

Wu, H.,Kaur, U.,Narlikar, G.J.,Cheng, Y.F. (登録日: 2024-06-17, 公開日: 2025-07-16, 最終更新日: 2025-07-30)

主引用文献

Kaur, U.,Wu, H.,Cheng, Y.,Narlikar, G.J.
Autoinhibition imposed by a large conformational switch of INO80 regulates nucleosome positioning.
Science, 389:eadr3831-eadr3831, 2025

PubMed Abstract: Increasing the flanking DNA from 40 to 80 base pairs (bp) causes ~100-fold faster nucleosome sliding by INO80. A prevalent hypothesis posits that the Arp8 module within INO80 enables a ruler-like activity. Using cryogenic electron microscopy, we show that on nucleosomes with 40 bp of flanking DNA, the Arp8 module rotates 180° away from the DNA. Deleting the Arp8 module enables rapid sliding irrespective of flanking DNA length. Thus, rather than enabling a ruler-like activity, the Arp8 module acts as a brake on INO80 remodeling when flanking DNA is short. This autoinhibition-based mechanism has broad implications for understanding how primitive nucleosome mobilization enzymes may have evolved into sophisticated remodelers.

PubMed: 40674492
DOI: 10.1126/science.adr3831

実験手法

ELECTRON MICROSCOPY (4.18 Å)