9CA1

Human TOP3B-TDRD3 core complex in DNA religation state

9CA1 の概要

• エントリーDOI: 10.2210/pdb9ca1/pdb
• EMDBエントリー: 45379
• 分子名称: DNA topoisomerase 3-beta-1, Tudor domain-containing protein 3, DNA (5'-D(P*TP*AP*CP*TP*AP*AP*A)-3'), ... (5 entities in total)
• 機能のキーワード: topoisomerase, dna, isomerase-dna complex, isomerase/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 89640.77

構造登録者

Yang, X.,Chen, X.,Yang, W.,Pommier, Y. (登録日: 2024-06-16, 公開日: 2024-07-17, 最終更新日: 2025-02-05)

主引用文献

Yang, X.,Chen, X.,Yang, W.,Pommier, Y.
Structural insights into human topoisomerase 3 beta DNA and RNA catalysis and nucleic acid gate dynamics.
Nat Commun, 16:834-834, 2025

PubMed Abstract: Type IA topoisomerases (TopoIAs) are present in all living organisms. They resolve DNA/RNA catenanes, knots and supercoils by breaking and rejoining single-stranded DNA/RNA segments and allowing the passage of another nucleic acid segment through the break. Topoisomerase III-β (TOP3B), the only RNA topoisomerase in metazoans, promotes R-loop disassembly and translation of mRNAs. Defects in TOP3B lead to severe neurological diseases. We present a series of cryo-EM structures of human TOP3B with its cofactor TDRD3 during cleavage and rejoining of DNA or RNA, thus elucidating the roles of divalent metal ions and key enzyme residues in each step of the catalytic cycle. We also obtained the structure of an open-gate configuration that addresses the long-standing question of the strand-passage mechanism. Our studies reveal how TOP3B catalyzes both DNA and RNA relaxation, while TOP3A acts only on DNA.

PubMed: 39828754
DOI: 10.1038/s41467-025-55959-y

実験手法

ELECTRON MICROSCOPY (3.26 Å)