9C8D

mouse Seipin/Adig complex

Summary for 9C8D

• Entry DOI: 10.2210/pdb9c8d/pdb
• EMDB information: 45301
• Descriptor: Seipin, Adipogenin (2 entities in total)
• Functional Keywords: seipin, adipogenin, lipid droplets, adipose, membrane protein
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 24
• Total formula weight: 741126.13

Authors

Li, C.,Han, Y.,Wynn, R.M.,Chen, Z.,Scherer, P.E. (deposition date: 2024-06-12, release date: 2025-08-27, Last modification date: 2025-11-19)

Primary citation

Li, C.,Sun, X.N.,Funcke, J.B.,Vanharanta, L.,Prasanna, X.,Gov, K.,Li, Y.,Virostek, M.,Joung, C.,Joffin, N.,Kanerva, K.,Szkalisity, A.,Kulig, W.,Straub, L.,Chen, S.,Velasco, J.,Cobb, A.,La Padula, D.,Wang, M.Y.,Onodera, T.,Voros, C.,Kim, D.S.,Kim, M.,Varlamov, O.,Li, Y.,Liu, C.,Nawrocki, A.R.,Zhao, S.,Oh, D.Y.,Wang, Z.V.,Gordillo, R.,Goodman, J.M.,Wynn, R.M.,Henne, W.M.,Vattulainen, I.,Han, Y.,Ikonen, E.,Scherer, P.E.
Adipogenin promotes the development of lipid droplets by binding a dodecameric seipin complex.
Science, 390:eadr9755-eadr9755, 2025

PubMed Abstract: The microprotein adipogenin (Adig) is predominantly expressed in adipose tissues. Here, we found that Adig interacts with seipin to form a stable, rigid complex. We present the structure of the seipin-Adig complex at an overall resolution of ~3.0 angstroms. The structure revealed that mammalian seipin assembles into two distinct oligomeric forms: undecamers and dodecamers. Adig selectively bound to the dodecameric form and enhanced seipin assembly by bridging and stabilizing adjacent subunits. Functionally, this complex promoted lipid droplet development at both early and late stages. In transgenic mice, adipocyte-specific overexpression of Adig increased fat mass and enlarged lipid droplets, whereas Adig deletion disrupted triglyceride accumulation in brown adipose tissues. Thus, Adig can modulate lipid storage through its structural and functional interactions with seipin.

PubMed: 41196993
DOI: 10.1126/science.adr9755

Experimental method

ELECTRON MICROSCOPY (2.98 Å)