9C8D

mouse Seipin/Adig complex

9C8D の概要

• エントリーDOI: 10.2210/pdb9c8d/pdb
• EMDBエントリー: 45301
• 分子名称: Seipin, Adipogenin (2 entities in total)
• 機能のキーワード: seipin, adipogenin, lipid droplets, adipose, membrane protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 741126.13

構造登録者

Li, C.,Han, Y.,Wynn, R.M.,Chen, Z.,Scherer, P.E. (登録日: 2024-06-12, 公開日: 2025-08-27, 最終更新日: 2025-11-19)

主引用文献

Li, C.,Sun, X.N.,Funcke, J.B.,Vanharanta, L.,Prasanna, X.,Gov, K.,Li, Y.,Virostek, M.,Joung, C.,Joffin, N.,Kanerva, K.,Szkalisity, A.,Kulig, W.,Straub, L.,Chen, S.,Velasco, J.,Cobb, A.,La Padula, D.,Wang, M.Y.,Onodera, T.,Voros, C.,Kim, D.S.,Kim, M.,Varlamov, O.,Li, Y.,Liu, C.,Nawrocki, A.R.,Zhao, S.,Oh, D.Y.,Wang, Z.V.,Gordillo, R.,Goodman, J.M.,Wynn, R.M.,Henne, W.M.,Vattulainen, I.,Han, Y.,Ikonen, E.,Scherer, P.E.
Adipogenin promotes the development of lipid droplets by binding a dodecameric seipin complex.
Science, 390:eadr9755-eadr9755, 2025

PubMed Abstract: The microprotein adipogenin (Adig) is predominantly expressed in adipose tissues. Here, we found that Adig interacts with seipin to form a stable, rigid complex. We present the structure of the seipin-Adig complex at an overall resolution of ~3.0 angstroms. The structure revealed that mammalian seipin assembles into two distinct oligomeric forms: undecamers and dodecamers. Adig selectively bound to the dodecameric form and enhanced seipin assembly by bridging and stabilizing adjacent subunits. Functionally, this complex promoted lipid droplet development at both early and late stages. In transgenic mice, adipocyte-specific overexpression of Adig increased fat mass and enlarged lipid droplets, whereas Adig deletion disrupted triglyceride accumulation in brown adipose tissues. Thus, Adig can modulate lipid storage through its structural and functional interactions with seipin.

PubMed: 41196993
DOI: 10.1126/science.adr9755

実験手法

ELECTRON MICROSCOPY (2.98 Å)