9C7V の概要
| エントリーDOI | 10.2210/pdb9c7v/pdb |
| EMDBエントリー | 45293 45294 45295 |
| 分子名称 | ER membrane protein complex subunit 10, Nicalin, BOS complex subunit NOMO2, ... (14 entities in total) |
| 機能のキーワード | membrane protein biogenesis, membrane protein complex, membrane protein |
| 由来する生物種 | Homo sapiens (human) 詳細 |
| タンパク質・核酸の鎖数 | 12 |
| 化学式量合計 | 530474.11 |
| 構造登録者 | |
| 主引用文献 | Page, K.R.,Nguyen, V.N.,Pleiner, T.,Tomaleri, G.P.,Wang, M.L.,Guna, A.,Hazu, M.,Wang, T.Y.,Chou, T.F.,Voorhees, R.M. Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins. Mol.Cell, 84:3302-, 2024 Cited by PubMed Abstract: Mammalian membrane proteins perform essential physiologic functions that rely on their accurate insertion and folding at the endoplasmic reticulum (ER). Using forward and arrayed genetic screens, we systematically studied the biogenesis of a panel of membrane proteins, including several G-protein-coupled receptors (GPCRs). We observed a central role for the insertase, the ER membrane protein complex (EMC), and developed a dual-guide approach to identify genetic modifiers of the EMC. We found that the back of Sec61 (BOS) complex, a component of the multipass translocon, was a physical and genetic interactor of the EMC. Functional and structural analysis of the EMC⋅BOS holocomplex showed that characteristics of a GPCR's soluble domain determine its biogenesis pathway. In contrast to prevailing models, no single insertase handles all substrates. We instead propose a unifying model for coordination between the EMC, the multipass translocon, and Sec61 for the biogenesis of diverse membrane proteins in human cells. PubMed: 39173640DOI: 10.1016/j.molcel.2024.08.005 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (6.6 Å) |
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