9C2T の概要
| エントリーDOI | 10.2210/pdb9c2t/pdb |
| EMDBエントリー | 45160 |
| 分子名称 | Capsid protein 2, Alpha-1-antichymotrypsin His-Pro-less (2 entities in total) |
| 機能のキーワード | b19, virion, capsid, viral protein |
| 由来する生物種 | Human parvovirus B19 詳細 |
| タンパク質・核酸の鎖数 | 7 |
| 化学式量合計 | 407209.67 |
| 構造登録者 | |
| 主引用文献 | Lee, H.,Assaraf, R.,Subramanian, S.,Goetschius, D.,Bieri, J.,DiNunno, N.M.,Leisi, R.,Bator, C.M.,Hafenstein, S.L.,Ros, C. Infectious parvovirus B19 circulates in the blood coated with active host protease inhibitors. Nat Commun, 15:9543-9543, 2024 Cited by PubMed Abstract: The lack of a permissive cell culture system has limited high-resolution structures of parvovirus B19 (B19V) to virus-like particles (VLPs). In this study, we present the atomic resolution structure (2.2 Å) of authentic B19V purified from a patient blood sample. There are significant differences compared to non-infectious VLPs. Most strikingly, two host protease inhibitors (PIs), inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) and serpinA3, were identified in complex with the capsids in all patient samples tested. The ITIH4 binds specifically to the icosahedral fivefold axis and serpinA3 occupies the twofold axis. The protein-coated virions remain infectious, and the capsid-associated PIs retain activity; however, upon virion interaction with target cells, the PIs dissociate from the capsid prior to viral entry. Our finding of an infectious virion shielded by bound host serum proteins suggests an evolutionarily favored phenomenon to evade immune surveillance and escape host protease activity. PubMed: 39500886DOI: 10.1038/s41467-024-53794-1 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.1 Å) |
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