9C1F の概要
| エントリーDOI | 10.2210/pdb9c1f/pdb |
| EMDBエントリー | 45117 |
| 分子名称 | Peptidyl-prolyl cis-trans isomerase FKBP1B, Ryanodine receptor 3, ZINC ION, ... (7 entities in total) |
| 機能のキーワード | ryr3, ryanodine receptor, calcium channel, ion channel, membrane protein |
| 由来する生物種 | Homo sapiens (human) 詳細 |
| タンパク質・核酸の鎖数 | 8 |
| 化学式量合計 | 2258025.72 |
| 構造登録者 | |
| 主引用文献 | Chen, Y.S.,Garcia-Castaneda, M.,Charalambous, M.,Rossi, D.,Sorrentino, V.,Van Petegem, F. Cryo-EM investigation of ryanodine receptor type 3. Nat Commun, 15:8630-8630, 2024 Cited by PubMed Abstract: Ryanodine Receptor isoform 3 (RyR3) is a large ion channel found in the endoplasmic reticulum membrane of many different cell types. Within the hippocampal region of the brain, it is found in dendritic spines and regulates synaptic plasticity. It controls myogenic tone in arteries and is upregulated in skeletal muscle in early development. RyR3 has a unique functional profile with a very high sensitivity to activating ligands, enabling high gain in Ca-induced Ca release. Here we solve high-resolution cryo-EM structures of RyR3 in non-activating and activating conditions, revealing structural transitions that occur during channel opening. Addition of activating ligands yields only open channels, indicating an intrinsically high open probability under these conditions. RyR3 has reduced binding affinity to the auxiliary protein FKBP12.6 due to several sequence variations in the binding interface. We map disease-associated sequence variants and binding sites for known pharmacological agents. The N-terminal region contains ligand binding sites for a putative chloride anion and ATP, both of which are targeted by sequence variants linked to epileptic encephalopathy. PubMed: 39366997DOI: 10.1038/s41467-024-52998-9 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.22 Å) |
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