9BSN

Structure of human K2P13.1 (THIK-1) in lipid nanodisc

9BSN の概要

• エントリーDOI: 10.2210/pdb9bsn/pdb
• EMDBエントリー: 44870
• 分子名称: Potassium channel subfamily K member 13, DODECANE, DECANE, ... (6 entities in total)
• 機能のキーワード: potassium channel, k2p, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82085.65

構造登録者

Roy-Chowdhury, S.,Minor, D.L. (登録日: 2024-05-13, 公開日: 2025-02-26, 最終更新日: 2025-05-14)

主引用文献

Roy-Chowdhury, S.,Jang, S.,Abderemane-Ali, F.,Naughton, F.,Grabe, M.,Minor Jr., D.L.
Structure of the human K 2P 13.1 channel reveals a hydrophilic pore restriction and lipid cofactor site.
Nat.Struct.Mol.Biol., 2025

PubMed Abstract: Polyunsaturated fatty acid (PUFA) lipids modulate the neuronal and microglial leak potassium channel K13.1 (THIK1) and other voltage-gated ion channel (VGIC) superfamily members through poorly understood mechanisms. Here we present cryo-electron microscopy structures of human THIK1 and mutants, revealing a unique two-chamber aqueous inner cavity obstructed by a hydrophilic barrier important for gating, the flow restrictor, and a P1-M4 intersubunit interface lipid at a site, the PUFA site, corresponding to the K small-molecule modulator pocket. This overlap, together with functional studies, indicates that PUFA site lipids are THIK1 cofactors. Comparison with a PUFA-responsive VGIC, K7.1, reveals a shared modulatory role for the pore domain intersubunit interface, providing a framework for understanding PUFA action on the VGIC superfamily. Our findings reveal the distinct THIK1 architecture, highlight the importance of the P1-M4 interface for K control by natural and synthetic ligands and should aid in the development of THIK subfamily modulators for neuroinflammation and autism.

PubMed: 40011746
DOI: 10.1038/s41594-024-01476-3

実験手法

ELECTRON MICROSCOPY (2.7 Å)