9BL8

MIT (L7C/A43C) Domain of Vps4p

Summary for 9BL8

Entry DOI	10.2210/pdb9bl8/pdb
Descriptor	Vacuolar protein sorting-associated protein 4, CHLORIDE ION (3 entities in total)
Functional Keywords	vps4 escrt escrtiii mit mim disulfide bond, protein transport
Biological source	Saccharomyces cerevisiae
Total number of polymer chains	1
Total formula weight	9555.18
Authors	Wienkers, H.J.,Han, H.,Whitby, F.G.,Hill, C.P. (deposition date: 2024-04-29, release date: 2025-03-12)
Primary citation	Wienkers, H.J.,Han, H.,Whitby, F.G.,Hill, C.P. Vps4 substrate binding and coupled mechanisms of Vps4p substrate recruitment and release from autoinhibition. Biorxiv, 2024 Cited by PubMed Abstract: The ESCRT pathway's AAA+ ATPase, Vps4p, remodels ESCRT-III complexes to drive membrane fission. Here, we use peptide binding assays to further the understanding of substrate specificity and the mechanism of autoinhibition. Our results reveal unexpected sequence preference to the substrate binding groove and an elegant mechanism of regulation that couples localization to substrate with release from autoinhibition. PubMed: 39282404 DOI: 10.1101/2024.09.07.611824 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.3 Å)

Structure validation