9BJE

Encapsulin 1 of a two-component Family 2B encapsulin shell from Streptomyces lydicus

9BJE の概要

• エントリーDOI: 10.2210/pdb9bje/pdb
• EMDBエントリー: 44632
• 分子名称: Crp/Fnr family transcriptional regulator (1 entity in total)
• 機能のキーワード: encapsulin, nanocompartment, virus like particle
• 由来する生物種: Streptomyces lydicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52256.59

構造登録者

Andreas, M.P.,Dutcher, C.,Giessen, T.W. (登録日: 2024-04-25, 公開日: 2025-04-30, 最終更新日: 2025-09-10)

主引用文献

Dutcher, C.A.,Andreas, M.P.,Giessen, T.W.
A Two-Component Pseudo-Icosahedral Protein Nanocompartment with Variable Shell Composition and Irregular Tiling.
Adv Sci, 12:e03617-e03617, 2025

PubMed Abstract: Protein shells or capsids are a widespread form of compartmentalization in nature. Viruses use protein capsids to protect and transport their genomes while many cellular organisms use protein shells for varied metabolic purposes. These protein-based compartments often exhibit icosahedral symmetry and consist of a small number of structural components with defined roles. Encapsulins are a prevalent protein-based compartmentalization strategy in prokaryotes. All encapsulins studied thus far consist of a single shell protein that adopts the viral Hong Kong 97 (HK97)-fold. Here, the characterization of a Family 2B two-component encapsulin from Streptomyces lydicus is reported. The differential assembly behavior of the two shell components and their ability to co-assemble into mixed shells with variable shell composition is demonstrated. The structures of both shell proteins are determined using cryo-electron microscopy. Using 3D-classification and cross-linking studies, the irregular tiling of mixed shells is highlighted. This work expands the known assembly modes of HK97-fold proteins and lays the foundation for future functional and engineering studies on two-component encapsulins.

PubMed: 40557621
DOI: 10.1002/advs.202503617

実験手法

ELECTRON MICROSCOPY (2.58 Å)