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9BGO

Pseudomonas phage DEV gp72 ejection protein (pre-ejection conformation)

9BGO の概要
エントリーDOI10.2210/pdb9bgo/pdb
EMDBエントリー44519
分子名称gp72 (1 entity in total)
機能のキーワードvirion scaffolding, complex, structural protein, gp72, ejection protein
由来する生物種Pseudomonas phage vB_PaeP_DEV
タンパク質・核酸の鎖数12
化学式量合計686198.86
構造登録者
Iglesias, S.M.,Hou, C.F.D.,Li, F.,Cingolani, G. (登録日: 2024-04-19, 公開日: 2024-09-04, 最終更新日: 2024-10-16)
主引用文献Lokareddy, R.K.,Hou, C.D.,Forti, F.,Iglesias, S.M.,Li, F.,Pavlenok, M.,Horner, D.S.,Niederweis, M.,Briani, F.,Cingolani, G.
Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor.
Nat Commun, 15:8482-8482, 2024
Cited by
PubMed Abstract: DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo-electron microscopy localized reconstruction, biochemical methods, and genetic knockouts. We built de novo structures of all capsid factors and tail components involved in host attachment. We demonstrate that DEV long tail fibers are essential for infection of Pseudomonas aeruginosa but dispensable for infecting mutants with a truncated lipopolysaccharide devoid of the O-antigen. We determine that DEV vRNAP is part of a three-gene operon conserved in 191 Schitoviridae genomes. We propose these three proteins are ejected into the host to form a genome ejection motor spanning the cell envelope. We posit that the design principles of the DEV ejection apparatus are conserved in all Schitoviridae.
PubMed: 39353939
DOI: 10.1038/s41467-024-52752-1
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (4.2 Å)
構造検証レポート
Validation report summary of 9bgo
検証レポート(詳細版)ダウンロードをダウンロード

236620

件を2025-05-28に公開中

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