9BFA

BCAT mutant

9BFA の概要

• エントリーDOI: 10.2210/pdb9bfa/pdb
• 関連するPDBエントリー: 8VXP
• 分子名称: Branched-chain-amino-acid aminotransferase, cytosolic, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: aminotransferase, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 164760.46

構造登録者

Dong, M. (登録日: 2024-04-17, 公開日: 2025-06-11)

主引用文献

Dare, E.S.,Newman, R.H.,Conway, M.E.,Dong, M.
Crystal structures of the phosphorylation mimics of human cytosolic branched chain aminotransferase.
Arch.Biochem.Biophys., 770:110479-110479, 2025

PubMed Abstract: The phosphorylation sites of the human cytosolic Branched Chain Aminotransferase (hBCATc) mediated by mitogen-activated protein kinase (MAPK)/extracellular-signal-regulated-kinase 2 (ERK2, also known as MAPK1) were mapped. The crystal structures of the phosphorylation mimics at T33 and T36 were determined. The modified transaminase activity of these variants was analyzed. Although there were no major conformational changes in the phosphorylation mimics of hBCAT, a regional conformational change at the interdomain loop was observed mainly in mutant T33E. Consistently, when the catalytic turnovers of the T33E and T36E mutants were comparable to the wild type of hBCATc, the K dropped significantly compared to the wild type, indicating a shift of the substrate binding affinity in the mutants. Taken together, this indicated the phosphorylation of hBCATc by ERK2 is affecting the hBCATc's transaminase activity.

PubMed: 40414328
DOI: 10.1016/j.abb.2025.110479

実験手法

X-RAY DIFFRACTION (1.786 Å)