9BDT

Apolipoprotein B 100 bound to LDL receptor and legobody

これはPDB形式変換不可エントリーです。

9BDT の概要

• エントリーDOI: 10.2210/pdb9bdt/pdb
• EMDBエントリー: 44469
• 分子名称: Apolipoprotein B-100, Legobody 8D3 Fab Heavy Chain, Legobody 8D3 Fab Light Chain, ... (9 entities in total)
• 機能のキーワード: ldl, apob100, ldl receptor, lipid transport
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 831615.50

構造登録者

Dearborn, A.D.,Reimund, M.,Graziano, G.,Lei, H.,Kumar, A.,Neufeld, E.B.,Remaley, A.T.,Marcotrigiano, J. (登録日: 2024-04-12, 公開日: 2024-12-25, 最終更新日: 2025-03-05)

主引用文献

Reimund, M.,Dearborn, A.D.,Graziano, G.,Lei, H.,Ciancone, A.M.,Kumar, A.,Holewinski, R.,Neufeld, E.B.,O'Reilly, F.J.,Remaley, A.T.,Marcotrigiano, J.
Structure of apolipoprotein B100 bound to the low-density lipoprotein receptor.
Nature, 638:829-835, 2025

PubMed Abstract: Apolipoprotein B100 (apoB100) is a structural component of low-density lipoprotein (LDL) and a ligand for the LDL receptor (LDLR). Mutations in apoB100 or in LDLR cause familial hypercholesterolaemia, an autosomal dominant disease that is characterized by a marked increase in LDL cholesterol (LDL-C) and a higher risk of cardiovascular disease. The structure of apoB100 on LDL and its interaction with LDLR are poorly understood. Here we present the cryo-electron microscopy structures of apoB100 on LDL bound to the LDLR and a nanobody complex, which can form a C-symmetric, higher-order complex. Using local refinement, we determined high-resolution structures of the interfaces between apoB100 and LDLR. One binding interface is formed between several small-ligand-binding modules of LDLR and a series of basic patches that are scattered along a β-belt formed by apoB100, encircling LDL. The other binding interface is formed between the β-propeller domain of LDLR and the N-terminal domain of apoB100. Our results reveal how both interfaces are involved in LDL dimer formation, and how LDLR cycles between LDL- and self-bound conformations. In addition, known mutations in either apoB100 or LDLR, associated with high levels of LDL-C, are located at the LDL-LDLR interface.

PubMed: 39663455
DOI: 10.1038/s41586-024-08223-0

実験手法

ELECTRON MICROSCOPY (5.4 Å)