9BCS

Intracellular domain of GC-A bound to ANP

9BCS の概要

• エントリーDOI: 10.2210/pdb9bcs/pdb
• EMDBエントリー: 44436
• 分子名称: Atrial natriuretic peptide receptor 1, ADENOSINE-5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: single pass transmembrane protein, guanylyl cyclase, atrial natriuretic peptide receptor, hypertension, membrane protein, lyase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 234556.07

構造登録者

Liu, S.,Huang, X. (登録日: 2024-04-09, 公開日: 2024-11-27, 最終更新日: 2025-03-26)

主引用文献

Liu, S.,Payne, A.M.,Wang, J.,Zhu, L.,Paknejad, N.,Eng, E.T.,Liu, W.,Miao, Y.,Hite, R.K.,Huang, X.Y.
Architecture and activation of single-pass transmembrane receptor guanylyl cyclase.
Nat.Struct.Mol.Biol., 32:469-478, 2025

PubMed Abstract: The heart, in addition to its primary role in blood circulation, functions as an endocrine organ by producing cardiac hormone natriuretic peptides. These hormones regulate blood pressure through the single-pass transmembrane receptor guanylyl cyclase A (GC-A), also known as natriuretic peptide receptor 1. The binding of the peptide hormones to the extracellular domain of the receptor activates the intracellular guanylyl cyclase domain of the receptor to produce the second messenger cyclic guanosine monophosphate. Despite their importance, the detailed architecture and domain interactions within full-length GC-A remain elusive. Here we present cryo-electron microscopy structures, functional analyses and molecular dynamics simulations of full-length human GC-A, in both the absence and the presence of atrial natriuretic peptide. The data reveal the architecture of full-length GC-A, highlighting the spatial arrangement of its various functional domains. This insight is crucial for understanding how different parts of the receptor interact and coordinate during activation. The study elucidates the molecular basis of how extracellular signals are transduced across the membrane to activate the intracellular guanylyl cyclase domain.

PubMed: 39543315
DOI: 10.1038/s41594-024-01426-z

実験手法

ELECTRON MICROSCOPY (4.4 Å)