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9B78

Mycobacterium tuberculosis CoaX Homohexamer

Summary for 9B78
Entry DOI10.2210/pdb9b78/pdb
EMDB information44303
DescriptorType III pantothenate kinase (1 entity in total)
Functional Keywordspantothenate kinase isoform, cytosolic protein
Biological sourceMycobacterium tuberculosis
Total number of polymer chains6
Total formula weight176050.07
Authors
Chen, J.,Ekiert, D.C.,Bhabha, G. (deposition date: 2024-03-27, release date: 2024-11-13, Last modification date: 2024-12-11)
Primary citationKahne, S.C.,Yoo, J.H.,Chen, J.,Nakedi, K.,Iyer, L.M.,Putzel, G.,Samhadaneh, N.M.,Pironti, A.,Aravind, L.,Ekiert, D.C.,Bhabha, G.,Rhee, K.Y.,Darwin, K.H.
Identification of a depupylation regulator for an essential enzyme in Mycobacterium tuberculosis.
Proc.Natl.Acad.Sci.USA, 121:e2407239121-e2407239121, 2024
Cited by
PubMed Abstract: In , proteins that are posttranslationally modified with a prokaryotic ubiquitin-like protein (Pup) can be degraded by bacterial proteasomes. A single Pup-ligase and depupylase shape the pupylome, but the mechanisms regulating their substrate specificity are incompletely understood. Here, we identified a depupylation regulator, a protein called CoaX, through its copurification with the depupylase Dop. CoaX is a pseudopantothenate kinase that showed evidence of binding to pantothenate, an essential nutrient synthesizes, but not its phosphorylation. In a ∆ mutant, pantothenate synthesis enzymes including PanB, a substrate of the Pup-proteasome system (PPS), were more abundant than in the parental strain. In vitro, CoaX specifically accelerated depupylation of Pup~PanB, while addition of pantothenate inhibited this reaction. In culture, media supplementation with pantothenate decreased PanB levels, which required CoaX. Collectively, we propose CoaX regulates PanB abundance in response to pantothenate levels by modulating its vulnerability to proteolysis by proteasomes.
PubMed: 39585979
DOI: 10.1073/pnas.2407239121
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.59 Å)
Structure validation

238582

數據於2025-07-09公開中

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