9B1E の概要
| エントリーDOI | 10.2210/pdb9b1e/pdb |
| EMDBエントリー | 44075 |
| 分子名称 | Helicase SWR1, Histone H3, Histone H4, ... (17 entities in total) |
| 機能のキーワード | chromatin remodeler, snf2 family atpase, histone exchange, h2a.z, gene regulation |
| 由来する生物種 | Drosophila melanogaster (fruit fly) 詳細 |
| タンパク質・核酸の鎖数 | 21 |
| 化学式量合計 | 1099122.27 |
| 構造登録者 | |
| 主引用文献 | Louder, R.K.,Park, G.,Ye, Z.,Cha, J.S.,Gardner, A.M.,Lei, Q.,Ranjan, A.,Hollmuller, E.,Stengel, F.,Pugh, B.F.,Wu, C. Molecular basis of global promoter sensing and nucleosome capture by the SWR1 chromatin remodeler. Cell, 2024 Cited by PubMed Abstract: The SWR1 chromatin remodeling complex is recruited to +1 nucleosomes downstream of transcription start sites of eukaryotic promoters, where it exchanges histone H2A for the specialized variant H2A.Z. Here, we use cryoelectron microscopy (cryo-EM) to resolve the structural basis of the SWR1 interaction with free DNA, revealing a distinct open conformation of the Swr1 ATPase that enables sliding from accessible DNA to nucleosomes. A complete structural model of the SWR1-nucleosome complex illustrates critical roles for Swc2 and Swc3 subunits in oriented nucleosome engagement by SWR1. Moreover, an extended DNA-binding α helix within the Swc3 subunit enables sensing of nucleosome linker length and is essential for SWR1-promoter-specific recruitment and activity. The previously unresolved N-SWR1 subcomplex forms a flexible extended structure, enabling multivalent recognition of acetylated histone tails by reader domains to further direct SWR1 toward the +1 nucleosome. Altogether, our findings provide a generalizable mechanism for promoter-specific targeting of chromatin and transcription complexes. PubMed: 39357520DOI: 10.1016/j.cell.2024.09.007 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (4.4 Å) |
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