9B0L

Cryo-EM structure of Acanthamoeba polyphaga mimivirus Fanzor2 ternary complex

Summary for 9B0L

• Entry DOI: 10.2210/pdb9b0l/pdb
• EMDB information: 44046
• Descriptor: DNA (5'-D(P*GP*AP*CP*GP*GP*TP*CP*GP*GP*GP*C)-3'), TnpB-like protein L79, DNA (5'-D(P*CP*CP*CP*A)-3'), ... (7 entities in total)
• Functional Keywords: transposon, fanzor, cryo-em, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• Biological source: Acanthamoeba polyphaga mimivirus; More
• Total number of polymer chains: 5
• Total formula weight: 151627.47

Authors

Qayyum, M.Z.,Schargel, R.D.,Tanwar, A.S.,Kellogg, E.H.,Kalathur, R.C. (deposition date: 2024-03-12, release date: 2024-10-09)

Primary citation

Schargel, R.D.,Qayyum, M.Z.,Tanwar, A.S.,Kalathur, R.C.,Kellogg, E.H.
Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily.
Nat.Struct.Mol.Biol., 2024

PubMed Abstract: RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases.

PubMed: 39354233
DOI: 10.1038/s41594-024-01394-4

Experimental method

ELECTRON MICROSCOPY (2.99 Å)