9B0L

Cryo-EM structure of Acanthamoeba polyphaga mimivirus Fanzor2 ternary complex

9B0L の概要

• エントリーDOI: 10.2210/pdb9b0l/pdb
• EMDBエントリー: 44046
• 分子名称: DNA (5'-D(P*GP*AP*CP*GP*GP*TP*CP*GP*GP*GP*C)-3'), TnpB-like protein L79, DNA (5'-D(P*CP*CP*CP*A)-3'), ... (7 entities in total)
• 機能のキーワード: transposon, fanzor, cryo-em, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• 由来する生物種: Acanthamoeba polyphaga mimivirus; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 151627.47

構造登録者

Qayyum, M.Z.,Schargel, R.D.,Tanwar, A.S.,Kellogg, E.H.,Kalathur, R.C. (登録日: 2024-03-12, 公開日: 2024-10-09, 最終更新日: 2025-06-04)

主引用文献

Schargel, R.D.,Qayyum, M.Z.,Tanwar, A.S.,Kalathur, R.C.,Kellogg, E.H.
Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily.
Nat.Struct.Mol.Biol., 32:243-246, 2025

PubMed Abstract: RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases.

PubMed: 39354233
DOI: 10.1038/s41594-024-01394-4

実験手法

ELECTRON MICROSCOPY (2.99 Å)