9AXU

Non-translating S. pombe ribosome large subunit

これはPDB形式変換不可エントリーです。

9AXU の概要

• エントリーDOI: 10.2210/pdb9axu/pdb
• EMDBエントリー: 43973
• 分子名称: Large ribosomal subunit protein eL42, Large ribosomal subunit protein eL6, Large ribosomal subunit protein uL30C, ... (45 entities in total)
• 機能のキーワード: non-translating, 60s, large subunit, s. pombe, schizosasccharomyces pombe, ribosome
• 由来する生物種: Schizosaccharomyces pombe (fission yeast); 詳細
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 2009666.38

構造登録者

Gluc, M.,Gemin, O.,Purdy, M.,Mattei, S.,Jomaa, A. (登録日: 2024-03-06, 公開日: 2024-10-16, 最終更新日: 2024-11-20)

主引用文献

Gemin, O.,Gluc, M.,Rosa, H.,Purdy, M.,Niemann, M.,Peskova, Y.,Mattei, S.,Jomaa, A.
Ribosomes hibernate on mitochondria during cellular stress.
Nat Commun, 15:8666-8666, 2024

PubMed Abstract: Cell survival under nutrient-deprived conditions relies on cells' ability to adapt their organelles and rewire their metabolic pathways. In yeast, glucose depletion induces a stress response mediated by mitochondrial fragmentation and sequestration of cytosolic ribosomes on mitochondria. This cellular adaptation promotes survival under harsh environmental conditions; however, the underlying mechanism of this response remains unknown. Here, we demonstrate that upon glucose depletion protein synthesis is halted. Cryo-electron microscopy structure of the ribosomes show that they are devoid of both tRNA and mRNA, and a subset of the particles depicted a conformational change in rRNA H69 that could prevent tRNA binding. Our in situ structural analyses reveal that the hibernating ribosomes tether to fragmented mitochondria and establish eukaryotic-specific, higher-order storage structures by assembling into oligomeric arrays on the mitochondrial surface. Notably, we show that hibernating ribosomes exclusively bind to the outer mitochondrial membrane via the small ribosomal subunit during cellular stress. We identify the ribosomal protein Cpc2/RACK1 as the molecule mediating ribosomal tethering to mitochondria. This study unveils the molecular mechanism connecting mitochondrial stress with the shutdown of protein synthesis and broadens our understanding of cellular responses to nutrient scarcity and cell quiescence.

PubMed: 39379376
DOI: 10.1038/s41467-024-52911-4

実験手法

ELECTRON MICROSCOPY (1.94 Å)