9AUR

Crystal structure of loop-closed A21 2'-OMe dumbbell RNA bridged by glycine

9AUR の概要

• エントリーDOI: 10.2210/pdb9aur/pdb
• 分子名称: Fab BL3-6 heavy chain, Fab BL3-6 light chain, Loop-closed A21 2'-OMe dumbbell RNA bridged by glycine, ... (5 entities in total)
• 機能のキーワード: rna aminoacylation, t-loop, glycine-bridged flexizyme, rna
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 54794.33

構造登録者

Radakovic, A.,Lewicka, A.,Todisco, M.,Aitken, H.R.M.,Weiss, Z.,Kim, S.,Bannan, A.,Piccirilli, J.A.,Szostak, J.W. (登録日: 2024-02-29, 公開日: 2024-09-04, 最終更新日: 2024-10-09)

主引用文献

Radakovic, A.,Lewicka, A.,Todisco, M.,Aitken, H.R.M.,Weiss, Z.,Kim, S.,Bannan, A.,Piccirilli, J.A.,Szostak, J.W.
A potential role for RNA aminoacylation prior to its role in peptide synthesis.
Proc.Natl.Acad.Sci.USA, 121:e2410206121-e2410206121, 2024

PubMed Abstract: Coded ribosomal peptide synthesis could not have evolved unless its sequence and amino acid-specific aminoacylated tRNA substrates already existed. We therefore wondered whether aminoacylated RNAs might have served some primordial function prior to their role in protein synthesis. Here, we show that specific RNA sequences can be nonenzymatically aminoacylated and ligated to produce amino acid-bridged stem-loop RNAs. We used deep sequencing to identify RNAs that undergo highly efficient glycine aminoacylation followed by loop-closing ligation. The crystal structure of one such glycine-bridged RNA hairpin reveals a compact internally stabilized structure with the same eponymous T-loop architecture that is found in many noncoding RNAs, including the modern tRNA. We demonstrate that the T-loop-assisted amino acid bridging of RNA oligonucleotides enables the rapid template-free assembly of a chimeric version of an aminoacyl-RNA synthetase ribozyme. We suggest that the primordial assembly of amino acid-bridged chimeric ribozymes provides a direct and facile route for the covalent incorporation of amino acids into RNA. A greater functionality of covalently incorporated amino acids could contribute to enhanced ribozyme catalysis, providing a driving force for the evolution of sequence and amino acid-specific aminoacyl-RNA synthetase ribozymes in the RNA World. The synthesis of specifically aminoacylated RNAs, an unlikely prospect for nonenzymatic reactions but a likely one for ribozymes, could have set the stage for the subsequent evolution of coded protein synthesis.

PubMed: 39178230
DOI: 10.1073/pnas.2410206121

実験手法

X-RAY DIFFRACTION (1.57 Å)